Immobilized enzyme for hydrolysis of N-glycans on glycoproteins in spin columns.
The PNGase F Immobilized Microspin columns contain PNGase F enzyme covalently coupled to agarose beads, for hydrolysis of N-glycans on glycoproteins with reduced enzyme peaks in the final sample.
The glycoprotein sample is incubated with PNGase F Immobilized in the spin column, and the deglycosylated protein is then easily collected by a centrifugation step. The activity of PNGase F on some glycoproteins can be slow or inhibited due to steric hindrance - longer incubation times or denaturation of the glycoprotein may in these cases be required.
The Microspin columns are available for hydrolysis of 5 or 10 x 0.2 mg glycoprotein.
•Easy-to-use spin columns
•Increased enzyme-to-substrate ratio for improved digestion efficiency
•Reduced enzyme peaks in the final sample
