Immobilized enzyme and affinity resin for below hinge digestion of IgG and purification of fragments. FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
FabRICATOR Fab2 Kit consists of spin columns of FabRICATOR Immobilized for antibody digestion, and spin columns of CaptureSelect™* Fc resin for affinity purification of F(ab’)2 and Fc fragments. One spin column contains sufficient FabRICATOR-coupled agarose beads to process 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) IgG.
FabRICATOR Fab2 Kit is available for digestion and purification of 0.5 mg - 100 mg antibody or Fc-fusion protein. It works on all subclasses of human, and some classes of monkey, rabbit and sheep IgG, but has limited activity on mouse IgG2a and IgG3 - for digestion and purification of these antibody species, we recommend using FabRICATOR Z Fab2 Kit (formerly FragIT Z kit).
•Purification of homogeneous F(ab’)2 fragments from several IgG species and subclasses
•Fast - pure fragments within 60-90 minutes
•Reliable workflow for preparative applications
