Immobilized enzyme on magnetic beads for automated digestion of IgG below the hinge. FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
The FabRICATOR MagIC magnetic beads contains FabRICATOR Immobilized enzyme. The beads enable fast automated digestion of multiple antibodies in parallel in a middle-level characterization workflow. They are designed for automation platforms, but perform equally well on a shaking table if automation is not available.
After digestion, the samples can be directly transferred to the autosampler of the LC-MS for fast chromatography or to another analytical method for analysis. Automated parallel antibody digestion and subunit generation allow for the processing of the numerous samples associated with clone selection, process development and stability studies for a potential biopharmaceutical. Automated antibody subunit analysis reduces the operator time and the risk for sample handling errors, and also increases the throughput.
•No enzyme in the final sample
•Fast, automated sample preparation
•Digestion of up to 96 samples in parallel
•Applicable to all magnetic automation platforms
