Lyophilized enzyme for hydrolysis of ɑ2-3-linked sialic acids.
SialEXO 2-3 Lyophilized is available as a lyophilized powder in 500 unit vials for desialylation of 0.5 mg glycoprotein.
In contrast to SialEXO, which is a sialidase mix, SialEXO 2-3 is an α2-3 specific sialidase, allowing targeted analysis of α2-3 linked sialic acids only. Efficient α2-3 desialylation of O- and N-glycosylated proteins can be achieved within 1 h.
SialEXO 2-3 hydrolyzes glycoproteins under native conditions and displays activity in pH ranging from 7.0 to 9.0.
The enzyme is derived from Akkermansia muciniphila and expressed in E. coli. The enzyme has a His-tag and the molecular weight is 66 kDa.
•Flexible format for method development
•Possible to combine enzymes for improved efficiency
•Amenable to automated workflows
•Ready to use – just add water!
